This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison
- mRNA binding
- Protein binding
Above are the two G.O. molecular functions of FMRP, in addition to the biological processes these two functions are very important in the formation of the spines in the dendrites with regulation of translation of other proteins.
Below you can see the protein domains of FMR1, FXR1, CYFIP1 and NUFIP1; the last two have domains that bind specifically to FMRP, and possibly help facilitate expression of other mRNAs [3].
Below is a hypothesized protein-protein interaction (PPI) of FMRP and CYFIP1 localized in the spines of the dendrites. As you can see when both FMRP and CYFIP1 form a complex their attachment to a mRNA prevents the translation of that protein. It can then be predicted that when either of these proteins is missing this mRNA might be constitutively expressed, which is often seen in FXS patients. To see more on this hypothesis please check out my future directions page, where I discuss the possibility of creating FXS similar phenotypes in mice by mutating other proteins such as CYFIP1.
References
[1] AmiGO: http://amigo.geneontology.org/cgi-bin/amigo/gp-assoc.cgi?gp=UniProtKB/Swiss-Prot:Q06787&session_id=6102amigo1269790532
[2] AmiGO Figures for FMR1
[3] Figure: Napoli, I., Mercaldo, V. et al. The Fragile X Syndrome Protein Represses Activity-Dependent Translation through CYFIP1, a New 4E-BP. 2008
[2] AmiGO Figures for FMR1
[3] Figure: Napoli, I., Mercaldo, V. et al. The Fragile X Syndrome Protein Represses Activity-Dependent Translation through CYFIP1, a New 4E-BP. 2008
Aime Agather
[email protected]
Last Updated 05/10/2010
Genetics 677
[email protected]
Last Updated 05/10/2010
Genetics 677